[All-postdocs] Bioseminar this Thursday, 12/10 - Despoina Konstantinou, Aristotle University of Thessaloniki
Margot McKlveen
mmcklveen at whoi.edu
Mon Dec 7 15:17:57 EST 2020
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*Biology Department Virtual Seminar*
*
Thursday, December 10 at Noon
Despoina Konstantinou, MSc, PhD candidate
Aristotle University of Thessaloniki, Greece
Sponge-cyanobacteria Associations in the Eastern Mediterranean Sea; a
Culture‐dependent Approach
The evolutionary and ecological success of sponges is thought to be
related to their capacity to host highly diverse and complex communities
of microbial symbionts. Cyanobacteria are among the most dominant and
important sponge symbionts, and their association with sponges has
attracted research interest from an ecological, evolutionary, and
biotechnological perspective. Our current knowledge for their diversity,
ecology and functioning is limited to few uncultivated taxa, while a
great part is “hidden” in metagenomics research studying the entire
microbial communities of sponges. The present study aimed to explore
sponge-cyanobacteria associations in a previously unexplored eastern
Mediterranean ecoregion, the Aegean Sea, by a culture-dependent
approach. This culture-dependent approach resulted in the isolation of
15 sponge-associated cyanobacterial strains from 12 sponge species.
Molecular, morphological and ecological data were obtained in order to
characterize the sponge-associated cyanobacterial strains. The analyses
of these data resulted in the description of six new genera and eleven
new species inside Cyanobacteria Phylum. The considerable number of
novel taxa characterized in this study highlighted the importance of
employing polyphasic culture-dependent approaches in order to reveal the
true sponge-associated cyanobacterial diversity. Further, the
sponge‐associated cyanobacterial strains were assessed for their
potential to produce bioactive compounds and found to be promising. In
order to better understand the biology of sponge-associated
cyanobacteria, two draft genomes of the most promising sponge-associated
strains were sequenced, and a comparative genomic analysis was
performed. Genome hallmarks for a symbiotic lifestyle were revealed
(such as evidence of genome reduction and detection of genes encoding
for eukaryotic-like proteins). Genome-mining analysis revealed natural
product biosynthetic gene clusters (BGCs) in sponge-associated
cyanobacterial genomes, suggesting that these strains deserve to be
further explored to reveal novel natural products.
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--
Margot McKlveen | she/her
Senior Administrative Assistant
Woods Hole Oceanographic Institution
Redfield Building Room 305 | MS 32
266 Woods Hole Rd.
Woods Hole, MA 02543
508-289-2334
mmcklveen at whoi.edu
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