<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Susan Shotwell has been sampling in Totten Inlet as well for some time, in the past couple years as part of the SoundTox program. <i>Akashiwo sanguinea</i> seems to be a common occurrence in that area, possibly with more intense blooms the last couple of years. In July of 2009, there was a fairly large bloom that was affecting the clam larvae Susan was trying to grow. <i>Akashiwo sanguinea</i> does have a cyst stage as well, so that may be contributing to the yearly blooms. Vegetative cells were grown from sediments from Esquimalt Bay in BC Canada in 1993 in one such study, at that time it was named <i>Gymnodinium sanguineam</i>.<div><br></div><div>Brian</div><div><br></div><div><div><div><div>On Oct 16, 2010, at 1:45 PM, Aimee Christy wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; font-family: 'Times New Roman'; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div lang="EN-US" link="blue" vlink="purple" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div class="WordSection1" style="page: WordSection1; "><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Thanks for the information. <o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Pacific Shellfish Institute has been collecting weekly phytoplankton samples from Totten Inlet from May to early September of this year. We began seeing Akashiwo at low concentrations (40,000 cells/L) on 7/7 and cell counts increased to 460,000 cells/L by 7/21. They remained at or near this concentration until peaking on 8/18 (684,000 cells/L). Dissipation seemed to occur around 9/8 (175,000 cells/L) when we collected our final sample for the season. It appears something triggered the initiation of another bloom….a sizeable one at that. <o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">In 2002-2003, we collected weekly plankton samples in Totten Inlet for a mussel raft project. Maximum Akashiwo counts for those 2 years were 268,000 cells/L (8/26/02) and 586,000 cells/L (8/7/03). <o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Hope this information is helpful.<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Aimee Christy<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Pacific Shellfish Institute <o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> <o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div><div style="border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; border-color: initial; border-top-style: solid; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding-top: 3pt; padding-right: 0in; padding-bottom: 0in; padding-left: 0in; "><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; ">From:</span></b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; "><span class="Apple-converted-space"> </span><a href="mailto:soundhab-bounces@whoi.edu" style="color: blue; text-decoration: underline; ">soundhab-bounces@whoi.edu</a><span class="Apple-converted-space"> </span>[mailto:soundhab-bounces@whoi.edu]<span class="Apple-converted-space"> </span><b>On Behalf Of<span class="Apple-converted-space"> </span></b>Brian Bill<br><b>Sent:</b><span class="Apple-converted-space"> </span>Friday, October 15, 2010 8:45 PM<br><b>To:</b><span class="Apple-converted-space"> </span><a href="mailto:soundhab@whoi.edu" style="color: blue; text-decoration: underline; ">soundhab@whoi.edu</a><br><b>Subject:</b><span class="Apple-converted-space"> </span>Re: SoundHAB: Possible Harmful Bloom in Totten Inlet<o:p></o:p></span></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">The dense bloom of marine algae in recent samples from Totten Inlet is<span class="Apple-converted-space"> </span><i>Akashiwo sanguinea</i>. This dinoflagellate was responsible for bird deaths on the outer Washington coast in late summer/fall 2009 due to the production of a surfactant like compound. Bird deaths were also attributed to<span class="Apple-converted-space"> </span><i>A. sanguinea</i> in Monterey Bay in 2007. <o:p></o:p></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">A cell count of the whole water sample was 660,000 cells/L.<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">On Oct 14, 2010, at 11:42 AM, Jack Rensel wrote:<o:p></o:p></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><br><br><o:p></o:p></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">Gordon King of Taylor Shellfish reports a very dense bloom of some kind of marine algae in portions of Totten Inlet presently. If any SoundHAB recipients are in the area and can sample, please take live or formalin preserved samples so we can identify the potential culprit. <o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">Also, several of you have asked about the HAB publication that I just produced with Tim Tynan and Nicky Haigh about Heterosigma affecting wild fish and salmon, so I attach it here for your use. This is pertinent to Totten Inlet as the Kennedy Creek Chum would normally be milling in the area at this time, with mean run timing of mid November.<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">Best regards,<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">Jack<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; "> <o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">J.E. Jack Rensel Ph.D.<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">Rensel Associates Aquatic Sciences<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">4209 234th Street N.E.<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">Arlington WA 98223 USA<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">360-435-3285<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; ">cell: 360-631-6538<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; "><a href="mailto:jackrensel@att.net" style="color: blue; text-decoration: underline; ">jackrensel@att.net</a><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; "><a href="http://www.AquaModel.org/" style="color: blue; text-decoration: underline; ">www.AquaModel.org</a><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><font class="Apple-style-span" face="Calibri, sans-serif" size="4"><span class="Apple-style-span" style="font-size: 15px;"><br></span></font></div></div></div></div></div></div></div></span></blockquote></div><br><div>
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: 'Times New Roman'; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: 'Times New Roman'; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: 'Times New Roman'; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">------------------------</span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">Brian D. Bill</span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">NOAA Fisheries</span></span></font></div><div><font class="Apple-style-span" face="'Lucida Grande'" size="3"><span class="Apple-style-span" style="font-size: 11px; ">Harmful Algal Blooms Program</span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">2725 Montlake Blvd E.</span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">Seattle, WA 98112</span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><br class="khtml-block-placeholder"></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">206-860-3387</span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">206-860-3335 fax</span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; "><a href="mailto:brian.d.bill@noaa.gov">brian.d.bill@noaa.gov</a></span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; "><a href="http://www.nwfsc.noaa.gov/hab/">www.nwfsc.noaa.gov/hab/</a></span></span></font></div><div><font class="Apple-style-span" face="Lucida Grande" size="3"><span class="Apple-style-span" style="font-size: 11px; font-family: 'Lucida Grande'; "><span class="Apple-style-span" style="font-family: 'Lucida Grande'; font-size: 11px; ">------------------------</span></span></font></div><div><br class="khtml-block-placeholder"></div><br class="Apple-interchange-newline"></span></div></span></span><br class="Apple-interchange-newline">
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