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<title>EukRef. Eukaryotic 18S rDNA curation workshop third edition. November 5 to 9, 2018. Roscoff, France. EXTENDED DEADLINE (Sept. 7)</title>
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<h2 style="text-align:justify;mso-line-height-alt:15.6pt"><span style="font-size:16.5pt">Join the curation effort and enroll in our third workshop! (EXTENDED DEADLINE Sept. 7)</span><o:p></o:p></h2>
<ul type="disc">
<li class="MsoNormal" style="color:#606060;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;line-height:15.6pt;mso-list:l0 level1 lfo1;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%">
<span style="font-size:11.5pt;font-family:"Helvetica","sans-serif"">What: Workshop to produce curated 18S rDNA reference database.<o:p></o:p></span></li><li class="MsoNormal" style="color:#606060;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;line-height:15.6pt;mso-list:l0 level1 lfo1;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%">
<span style="font-size:11.5pt;font-family:"Helvetica","sans-serif"">When: <strong>
<span style="font-family:"Helvetica","sans-serif"">November 5th – 9th, 2018</span></strong>.<o:p></o:p></span></li><li class="MsoNormal" style="color:#606060;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;line-height:15.6pt;mso-list:l0 level1 lfo1;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%">
<span style="font-size:11.5pt;font-family:"Helvetica","sans-serif"">Where: <strong>
<span style="font-family:"Helvetica","sans-serif"">Roscoff</span></strong>, France at the
<strong><span style="font-family:"Helvetica","sans-serif""><a href="https://eukref.us10.list-manage.com/track/click?u=7289b238b188944762ff1307e&id=fc1fd1d64e&e=98677ccf8e" target="_blank"><span style="color:#6DC6DD;font-weight:normal">Station Biologique de
Roscoff</span></a>.</span></strong><o:p></o:p></span></li><li class="MsoNormal" style="color:#606060;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;line-height:15.6pt;mso-list:l0 level1 lfo1;-ms-text-size-adjust: 100%;-webkit-text-size-adjust: 100%">
<span style="font-size:11.5pt;font-family:"Helvetica","sans-serif"">How much: We will cover the cost of housing, breakfast, and lunch, and reimburse for the cost of flights up to $400 for participants within Europe and $1500 for those from the rest of the world.
Thanks to the generous contribution of the <a href="https://eukref.us10.list-manage.com/track/click?u=7289b238b188944762ff1307e&id=bacc353fe2&e=98677ccf8e" target="_blank"><span style="color:#6DC6DD">Moore Foundation</span></a>.<o:p></o:p></span></li></ul>
<p class="MsoNormal" style="text-align:justify;line-height:150%"><span style="font-size:11.5pt;line-height:150%;font-family:"Helvetica","sans-serif";color:#606060">We would like you to join us this fall in our third workshop that will take place in Roscoff,
France from November 5 to 9. This effort brings together taxonomists with expertise in individual lineages that span the eukaryotic tree of life to curate reference 18S rDNA sequences of these lineages by incorporating knowledge of phylogenetic, morphological,
and/or environmental contextual data. During the workshop, the working group will integrate the curation efforts on individual eukaryotic groups into a biological data warehouse consisting of curated sequences, flexible taxonomy, and phylogenetic trees and
their underlying sequence alignments. We will further use this 18S rDNA reference database to investigate the environmental distribution of eukaryotic microbes from large-scale HTES datasets. Each curator is encouraged to use their curated data to address
research questions of interest. We will discuss how to tackle such projects and encourage participants to publish their results. If you are a PhD student or a Postdoc we encourage you to apply before September the 7th.<br>
<o:p></o:p></span></p>
<h4 align="center" style="text-align:center;mso-line-height-alt:15.6pt"><strong><span style="font-size:18.0pt;font-family:"Helvetica","sans-serif"">Application deadline: September 7th 2018</span></strong><o:p></o:p></h4>
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<p class="MsoNormal" align="center" style="text-align:center"><span style="font-family:"Arial","sans-serif""><a href="https://eukref.us10.list-manage.com/track/click?u=7289b238b188944762ff1307e&id=5ed94bc7df&e=98677ccf8e" target="_blank" title="Apply
here!"><b><span style="color:white;text-decoration:none">Apply
here!</span></b></a> <o:p></o:p></span></p>
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<p class="MsoNormal"><img border="0" width="528" id="_x0000_i1031" src="https://gallery.mailchimp.com/7289b238b188944762ff1307e/images/17e1c318-7f96-43bf-82b7-c298c4f6abfc.png"><o:p></o:p></p>
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<h2 style="text-align:justify;mso-line-height-alt:15.6pt"><strong><span style="font-family:"Helvetica","sans-serif"">EukRef</span></strong><o:p></o:p></h2>
<h3 style="text-align:justify;line-height:15.6pt"><strong><span style="font-family:"Helvetica","sans-serif"">Phylogenetically informed curation of Eukaryotic 18S rDNA</span></strong><o:p></o:p></h3>
<p class="MsoNormal" style="mso-margin-top-alt:12.0pt;margin-right:0cm;margin-bottom:12.0pt;margin-left:0cm;text-align:justify;line-height:15.6pt;-ms-text-size-adjust:
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<span style="font-size:11.5pt;font-family:"Helvetica","sans-serif";color:#606060">The diversity of eukaryotes extends far beyond the familiar plants, animals, and fungi. In fact, the vast majority of eukaryotic lineages are microbial. Eukaryotic microbes (protists)
are important players in ecological processes and also directly influence the biology and health of animals and plants as parasites, commensals and symbionts. However, the extent of their diversity is still largely unknown because most eukaryotes have not
yet been or cannot be cultured.<br>
<br>
High-throughput environmental sequencing (HTES) has greatly expanded our understanding of microbial biodiversity and its ecological role. HTES enables characterization of microbial communities rapidly and from hundreds of samples at the same time. The depth
of sampling has also revealed novel diversity in all ecosystems examined to date. However, the value of HTES data for cataloging the extent and distribution of protistan biodiversity is critically dependent on the quality of the reference databases used to
annotate these sequences.<br>
<br>
There is a growing and urgent need for well-curated reference databases to annotate the flood of environmental sequences coming in. The lack of a successful method to clean up mislabeled sequences makes manual curation by experts a necessary task. Ribosomal
DNA is the marker most frequently used to characterize diversity because it is universally present and has been sequenced for the most comprehensive array of known taxa (microscopically identified and/or cultured organisms). Curated eukaryotic databases of
ribosomal DNA have greatly improved analysis capacity in recent years. However, they struggle to keep pace with rapidly changing views on eukaryotic taxonomy, the influx of new data, and computational challenges related to assembling high quality alignments
and trees that are necessary for accurate characterization of lineage diversity. As new environmental sequence data continues to reveal novel lineages these data should ideally inform refinements in taxonomy and be incorporated into reference databases. This
rarely happens in practice because 1) the communities building taxonomic frameworks for eukaryotes are distinct from those conducting environmental sequence analysis, and 2) curating the vast amounts of existing data in a phylogenetic framework is beyond the
scope of individual research groups. Investment now in a curated reference database with high quality alignments and phylogenetic trees will pay dividends now through the diversity research it will enable and in the future because it will facilitate easier
and more reliable maintenance and automatic growth that can keep pace with developments in the field.<br>
<br>
Generating a phylogenetically and taxonomically informed reference databases in and of itself leads to novel insights into microbial diversity and ecology, in addition to producing a community resource that adds value to further investigations. Many sequences
across the eukaryotic tree of life are poorly or misannotated and have not been assembled into a comprehensive and coherent phylogenetic framework. As a result, the curation process can uncover additional novel lineages, refine understanding of the relationships
among environmental clades and previously described lineages, and offer new glimpses into the diversity contained within these clades.<o:p></o:p></span></p>
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