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id="docs-internal-guid-88a24c48-7fff-2960-995c-947b29be4321">
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style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Biology Department Virtual Seminar</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Thursday, May 27 at Noon</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Zoom link: </span><a
href="https://whoi-edu.zoom.us/j/93395960738?pwd=Y0VtaFh5SG9vcU1uSWJVOU1xZFEyQT09"
style="text-decoration:none;"><span style="font-size:11pt;font-family:Arial;color:#1155cc;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:underline;-webkit-text-decoration-skip:none;text-decoration-skip-ink:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">https://whoi-edu.zoom.us/j/93395960738?pwd=Y0VtaFh5SG9vcU1uSWJVOU1xZFEyQT09</span></a></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:700;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">MIT-WHOI Joint Program students</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:700;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Arianna Krinos</span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">: “Eukrhythmic: leveraging the metatranscriptomic landscape to reproducibly detect and describe marine protistan communities”</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:700;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Ciara Willis</span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">: “Comparing estimates of larval dispersal in a coral reef fish derived from a coupled biophysical model and DNA parentage analyses”</span></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:700;font-style:normal;font-variant:normal;text-decoration:underline;-webkit-text-decoration-skip:none;text-decoration-skip-ink:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Arianna Krinos - Eukrhythmic: leveraging the metatranscriptomic landscape to reproducibly detect and describe marine protistan communities</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Metatranscriptome analysis, the processing and interpretation of transcribed sequences from a biological community, has emerged as a promising approach to answer questions about natural microbial communities without prior knowledge or bias. Metatranscriptomes are an accessible means to characterize a more complete suite of expressed genes. Despite the potential of this technology, processing methods are relatively new, and often focused on RNAseq data from single-organism transcriptomes or prokaryotic communities, not mixed eukaryotic systems. As more metatranscriptomes are collected in oceanographic surveys, it is crucial to generate metatranscriptome-specific tools to improve study reproducibility and comparability. Here, we introduce eukrhythmic, a Snakemake-based metatranscriptome processing pipeline designed to address common problems in analyzing marine or other environmental samples. Using multiple assembly methods and tandem sample processing, eukrhythmic minimizes assembly bias, and enables rapid collation of samples from multiple sites. The pipeline enables functional and taxonomic annotation with special consideration of microbial eukaryotes, and provides a curated assembly that can be integrated with metaproteomic data. In particular, we show how the pipeline can be applied to understand a diel metatranscriptomic time series from the Western Antarctic Peninsula, and emphasize how metatranscriptomics can provide deep insight into the diversity and ecology of these samples.</span></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:700;font-style:normal;font-variant:normal;text-decoration:underline;-webkit-text-decoration-skip:none;text-decoration-skip-ink:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Ciara Willis - Comparing estimates of larval dispersal in a coral reef fish derived from a coupled biophysical model and DNA parentage analyses</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">The dispersal of pelagic larvae is a defining feature of the life histories of many marine species, including coral reef fishes. Resolving the dispersal of these larvae and the resulting connectivity of reef fish populations is a major focus in ocean ecology and is of critical importance to the effective design and implementation of marine reserve networks. Larval surveys, population genetics, hydrodynamic modelling, and tagging have all been used to infer population connectivity among reefs, with varying degrees of success. Development of DNA parentage analyses has produced direct estimates of population connectivity in reef fishes but with considerable logistical investment in field and lab work. Coupled biophysical modelling can potentially be used to track virtual larvae of any reef fish species but requires a number of assumptions that remain to be tested. Here, I leverage an existing DNA parentage dataset to test the skill of a coupled biophysical model to estimate larval dispersal of the vagabond butterflyfish (Chaetodon vagabundus) in Kimbe Bay, PNG. I used an embedded particle tracking module in the Finite Volume Coastal Ocean Model (FVCOM) system to estimate dispersal of virtual butterflyfish larvae with simplistic behaviour and spatially invariant mortality. The rare opportunity to test biophysical model results against empirical data is of value not just to future work in Kimbe Bay, but also for future use in sites where field sampling is cost-prohibitive or logistically difficult.</span></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Join Zoom Meeting</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><a
href="https://whoi-edu.zoom.us/j/93395960738?pwd=Y0VtaFh5SG9vcU1uSWJVOU1xZFEyQT09"
style="text-decoration:none;"><span style="font-size:11pt;font-family:Arial;color:#1155cc;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:underline;-webkit-text-decoration-skip:none;text-decoration-skip-ink:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">https://whoi-edu.zoom.us/j/93395960738?pwd=Y0VtaFh5SG9vcU1uSWJVOU1xZFEyQT09</span></a></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Meeting ID: 933 9596 0738</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Passcode: 6x!yxT</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">One tap mobile</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+13126266799,,93395960738#,,,,*470118# US (Chicago)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+16465588656,,93395960738#,,,,*470118# US (New York)</span></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Dial by your location</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><span class="Apple-tab-span" style="white-space:pre;"> </span></span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+1 312 626 6799 US (Chicago)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><span class="Apple-tab-span" style="white-space:pre;"> </span></span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+1 646 558 8656 US (New York)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><span class="Apple-tab-span" style="white-space:pre;"> </span></span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+1 301 715 8592 US (Washington DC)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><span class="Apple-tab-span" style="white-space:pre;"> </span></span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+1 346 248 7799 US (Houston)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><span class="Apple-tab-span" style="white-space:pre;"> </span></span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+1 669 900 6833 US (San Jose)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><span class="Apple-tab-span" style="white-space:pre;"> </span></span><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">+1 253 215 8782 US (Tacoma)</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Meeting ID: 933 9596 0738</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Passcode: 470118</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Find your local number:</span><a
href="https://whoi-edu.zoom.us/u/adHtj1IkPp"
style="text-decoration:none;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"> </span><span style="font-size:11pt;font-family:Arial;color:#1155cc;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:underline;-webkit-text-decoration-skip:none;text-decoration-skip-ink:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">https://whoi-edu.zoom.us/u/adHtj1IkPp</span></a></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Join by SIP</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;"><a class="moz-txt-link-abbreviated" href="mailto:93395960738@128.128.247.81">93395960738@128.128.247.81</a></span></p>
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<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Join by H.323</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">128.128.247.81</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Meeting ID: 933 9596 0738</span></p>
<p dir="ltr"
style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;background-color:transparent;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;white-space:pre;white-space:pre-wrap;">Passcode: 470118 </span></p>
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<pre class="moz-signature" cols="72">--
Margot McKlveen | she/her
Senior Administrative Assistant
Woods Hole Oceanographic Institution
Redfield Building Room 305 | MS 32
266 Woods Hole Rd.
Woods Hole, MA 02543
508-289-2334
<a class="moz-txt-link-abbreviated" href="mailto:mmcklveen@whoi.edu">mmcklveen@whoi.edu</a></pre>
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